Message Us With Any Questions Dismiss, THE ULTIMATE 2022 FESTIVAL SURVIVAL GUIDE, Marquis reagent: How does it work and why is it useful for testing drugs. 7 (1975) 563. 83 (1975) 70. As this precursor became harder to find, its possible that unscrupulous precursor vendors sold fennel oil, which smells similar to safrole oil, to black-market labs looking to synthesize MDMA. //]]>. Simon's reagent is a 2 part test, one part on its own is orange and the other colorless. Consider upgrading to the Complete Test Kit to narrow down what your sample is. D. B. Fishbach, Chemistry and Physics of Carbon, edited by P. L. Walker Jr, Vol. Test strips work great. All Rights Reserved. It may have a slightly more olive-green color before turning purple black, but its generally regarded that marquis,mecke and mandelin turn a similar color when testing pure MDMA within 60 seconds (a dark black), If your molly turns yellow with Mecke, you can have a strong suspicion that you may have been sold a bath salt like methylone or butylone, since these cathinones turn a yellowish collor with mecke, instead of olive-black like MDMA should. If youre testing blotters, use 1/6th of a blotter.2. Simon's reagent is composed of an aqueous mixture of sodium nitroprusside, sodium carbonate and acetaldehyde, which is dripped onto the substance being tested. doi:10.1371/journal.pone.0069609. This modification is not included in the UN Precursor Test Kit and other spot tests alike. We have a live chat on our website, where you can talk to us incase you need any assistance when testing or have any questions. This doesnt just mean selling them cheaper than everyone else, though. If your sample consists of MDMA or MDE, it will turn a blue color. Soc. citrate utilisation) produces an alkaline reaction and changes the color of the medium from green to bright blue.Examples:Serratia and the majority of the Enterobacter, Citrobacter, Klebsiella, Proteus and Providencia species, except Morganella morganii and Klebsiella rhinoscleromatis. / Telegram / Signal / WhatsApp: These carbons are characterized by X-ray diffraction (d %PDF-1.5 % The oxidation was carried out in the apparatus shown in Fig. Subscribe for new videos - Thanks for keeping it real.Reagent Kit Used: http://bunkpolice.com/simons-reagent-kit/USING THIS VIDEO FOR OTHER TEST KITS MAY PRO. 0000004461 00000 n Tel. 0000001811 00000 n only test performed on substances and be used as evidence for conviction. Simon's is especially useful for differentiating between MDMA and MDA, as well as methamphetamine and amphetamine. Replace the cap. Preparation of Benedict's Reagent. Separate storage of the aldehyde and base are necessary to prevent aldol polymerisation of the aldehyde. Dancesafe. Well one of the classic precursors in MDMA synthesis is safrole oil. 0000003976 00000 n The reactions of this medium alone are not sufcient for identication to the species level. These were excellent and worked very precisely seeing I have no previous lab skills. Created October 2011. Ltd.-Simmons Citrate Agar (59437). Identification of Drugs of Abuse, NIJ Standard 0604.01, National 0000008574 00000 n Squeeze TWO(2) DROPS of Sb out of the bottle onto the same powder/liquid already on the plate from step 2. Simon's reagent is used as a simple spot-test to presumptively identify alkaloids as well as other compounds. And while PMA is rare in the US, was reported to be seen as recently as the year 2001 in a Mitsubishi pill sold as MDMA. Positive growth (i.e. Lastly, we have Simons reagent. 0000002020 00000 n Find the best reagent test kits HERE. //]]> That drug is PMA/PMMA. Simon's Reagent: Solution A: Dissolve 1 g of sodium nitroprusside in 50 mL of distilled water and add 2 mL of acetaldehyde to the solution with thorough mixing. 53 (1970) 1126. 0000020961 00000 n Uses [ edit] February 1989. You've just added this product to the cart: Copyright DanceSafe, Inc 1999 - 2023. modified 7th November 2014. 1 (1976) 391. TestSimply remove the cap from your bottle and tilt it until a drop falls out.From the moment the testkit liquid hits the sample, watch closely for 30-60 seconds for any colour changes.Tip: Many samples will have an initial colour change followed by another over time, so its important to keep a close eye on them.Make sure that the sample doesnt touch the bottle tip.3. NOTE: Simons can only test the PRESENCE, not PURITY or QUANTITY of a specific substance. Growth; color change of medium to blueNegative control:Escherichia coli ATCC 25922. https://doi.org/10.1007/BF00540628. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); About Us - Contact Us - Privacy Policy & Disclaimer, Benedicts Test- Principle, Composition,, Widal Test- Introduction, Principle, Procedure,, Different Size, Shape and Arrangement of Bacterial Cells, Gram Staining: Principle, Procedure, Interpretation,, Nutrient Agar: Composition, Preparation and Uses, MacConkey Agar- Composition, Principle, Uses,, Catalase Test- Principle, Uses, Procedure, Result, Citrate Utilization Test- Principle, Media, Procedure and Result, Salmonella Shigella (SS) Agar- Composition, Principle, Uses, Preparation and Result Interpretation, Malonate Test Principle, Procedure, Uses and Interpretation. Dalynn Biologicals- SIMMONS CITRATE AGAR (TS62). Always test under a strong light and using the smallest possible sample. If so, register here for discount pricing. Delivery is 2-5 days depending on location. Simons should indeed not react to MDA, so that discoloration will be because of impurities. Your email address will not be published. 0000015052 00000 n More info. 0000009378 00000 n 0000213720 00000 n This includes the cathinones and presumably K-2C-x compounds. Includes one freefentanyl test stripand one freeamphetamine test strip. Get the latest business insights from Dun & Bradstreet. Faculty of Technology, Gunma University, Kiryu, 376, Gunma, Japan, Northern Carbon Research Laboratories, School of Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne, UK, You can also search for this author in Chem. It is one of three reagents in the MDMA kit. 7 (Marcel Dekker, New York, 1971) p. 83. They usually form a deeper blue color, and often a grey-ish precipitate. World Customs Organisation (WCO) Illicit Trade Reports, Cobalt The uninoculated medium will be a deep forest green due to the pH of the sample and the bromothymol blue. These cookies do not store any personal information. <<41913147f5e7104eaf4d28b86439ae3e>]>> Secondly use reagent bottle Sb. PubMedGoogle Scholar, ya, A., Ida, Y., Takabatake, M. et al. Unlike other Simons A/B Reagent Providers we provide you with everything you need to identify illicit substances and compounds. A characteristic blue coloration appears when the test is positive. Using the test tube place one drop of RobaTest reagent and then 2 of buffer and swirl gently (to mix) and wait 30 to 60 seconds and note the colour. However, a slow transition of the initial color into yellow, followed by an orange-brown color can be observed with both compounds, thus indicating the instability of the complexes initially formed, and an obvious decomposition of the two compounds under the alkaline conditions of this color reaction. regularly use illicit substances and can be considered dependent, Provided by the Springer Nature SharedIt content-sharing initiative, Over 10 million scientific documents at your fingertips, Not logged in no citrate utilisation) the color of the medium remains unchanged.Examples:Escherichia coli,Shigella, Yersinia, Edwardsiella species, etc. Bacteriological agar is thesolidifying agent. This website uses cookies to improve your experience while you navigate through the website. Cu(ephedrine)2 complex, Bull. High Press. The Ts- and G-components react faster than other carbons in Simon's reagent but react slower in mixed acids. Lower your dose with a DanceSafe branded "Less is More" pill cutter. 0000000016 00000 n They must be used together for the reaction to work. The amine moiety of the alkaloids reacts with acetaldehyde to yield an enamine, which subsequently . Immediately observe the color change and compare it with Simons color chart. The Simon's reagent reacts preferentially with the more graphitic structures of the G-component unlike the Ts-component which appears to be structurally homogeneous. Right here we have great insight into why Marquis reagent is so versatile because we can use it to differentiate between 3 of the most common drugs sold as molly. Kovar, [4], Last edited on 28 February 2023, at 18:34, Journal of the American Pharmaceutical Association, "Color Tests for Precursor Chemicals of Amphetamine-Type Substances", https://en.wikipedia.org/w/index.php?title=Chen-Kao_reaction&oldid=1142137153, Pale blue precipitate (the crystals in the precipitate are violet), Pale blue precipitate, turns to violet, through grey (greenish) in 2-3 minutes it turns to orange(brownish), Bright blue precipitate, through green it turns to brown, after 10 minutes the precipitate and the solution are orange, Precipitate forming a ring between layers, Bright blue, after 5 min it turns to grey (brownish sediment). Subscribe and get 10% off your first order. 13 (1975) 353. The medium may be used either as slopes in test tubes or as a plate medium in petri dishes. solution, Journal of the American Pharmaceutical Association Volume 19, Our contact info in case you need us 24/7 for anything, have any issues, comments, questions, concerns. individual tests worth of products distributed in the last year. 0000002305 00000 n 109 (1977) 145. 0000026161 00000 n This post should give you a good broad overview for why we include Marquis, Mecke, Mandelin and Simons reagent in our MDMA test kit package, and now you even understand some of the chemistry behind some of these tests as well. However, it is known from previously published cross-testing work that various pharmaceuticals not related to the ephedrine group may produce similar blue copper complexes. So every drug that Simon's reacts with will theoretically have a secondary amine group. 0000003097 00000 n It is one of three reagents in the MDMA kit. 1. . I would like to go through test-by-test and describe our personal recommendations for molly testing reagents and explain why we recommend each. Magnesium Sulfate is a cofactor for a variety of metabolic reactions. Jpn., (1964), 37, 1363. Scroll or click "accept" to accept and continue. They can detect all adulterants at once, but still require reagent tests for identifying ingredients. 17 (1979) 131. Simon's A/B Reagent. Instructions, safe handling, storage and most importantly reactions charts. These labs then unknowingly synthesize PMA/PMMA, and the rest is history. Phone: (402) 235-9889 Email: info(at)wimscilabs.com 0000125387 00000 n 1 (1972) 54. Your email address will not be published. DoseTest is here to shake up the harm reduction world, taking lifesaving materials like reagent test kits and fentanyl test strips from the small niche where they have lived and making them accessible to everyone. In the early 1920s, Koser developed a liquid medium formulation for the differentiation of fecal coliforms from the coliform group. WIM Scientific Laboratories makes use of superior laboratory chemicals and extensive attention to detail, ensuring . 0000005043 00000 n First and foremost they care about your general safety and take that into consideration. The presence of MDMA/MDE/Methamphetamine/methylone will be indicated by a change to a bright cobalt blue. Starting from the basics, reagent testing kits are chemical mixtures that react with a given compound(drug) such as MDMA, and produce a predictable color when they come in contact with that drug. Skip to the content Call Today: (402) 235-9889Spreading Education and Safety Information. DoseTest is a multi-national company, so we have distribution from both Europe & the United States. No reaction within the first 30 seconds or unexpected results indicate the sample doesnt contain the expected substance or is adulterated. To see if your test-kit is still valid after long term storage test common substances like Vitamin C, Aspirin, Ibuprofen, Paracetamol or any known substances (i.e an sample you know for sure is a certain substance) and see if the reaction is correct in reference to our reagent tool. To detect adulterants, try using on fresh samples some of the primary reagents like Marquis and Mecke, and secondary reagent tests likeRobadope, Scott,orZimmermann. Here, the copper (II) sulfate acts as a source of Cu 2+ ions, the sodium . Issue 12, pages 1292-1299, December 1930. be the 1520 52 Ltd.-Simmons Citrate Agar (M099). My Account Shipping FAQs Menu My Account Shipping FAQs Home Testing Kits 113 (1978) 1. 20 (1970) 133. 10 (1972) 65. bottom of a mug or a plate) prepare a sample no bigger than this size -> . //stream 37 (1958) 323. Chem. (see Table III)[4], When acidic solution of ephedrine or pseudoephedrine is mixed with copper sulfate and sodium hydroxide solutions, a violet color is formed. Simon's reagent was prepared as a solution of 5g silver dichromate in 25cm 3 concentrated sulphuric acid. However, MDA is a primary amine, and MDMA is a secondary amine. So, if marquis turned orange, and mecke doesnt change color at all, you have another confirmation that you have some type of amphetamine instead of MDMA. On a clean ceramic surface (e.g. All orders ship same day if placed before 12PM CST. PreparePrepare a tiny bit of your drug on a white ceramic plate or mug.Tip: You should use the same amount of powder as on a matchstick head, or a grain of rice. forensic science services, SCIENTIFIC Well, we have already covered most of the common drugs that are sold as molly: MDMA, amphetamine, and bath salts(methylone/butylone etc. Crystallogr. A good question would be why do people sell a deadly drug as MDMA? The Simons Reagent kit is a secondary test (after the use of Marquis, Mecke, & Mandelin) that tells the difference between MDMA vs MDA, methamphetamine vs amphetamine, or between MDPV vs methylone. G. Norwitz and M. Galan, ibid. Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. Precursor Chemicals of Amphetamine-Type Substances. Carbon preparation Non-graphitized carbon (A-component) was pre- pared from a phenolic resin (phenol formaldehyde, PF) [10], cured at 80 ~ C for 1 day, by carboniz- . ), however one other important drug we didnt mention is MDA (also known as sass or sassafras). 0000002179 00000 n c), high-resolution phase-contrast electron microscopy, Raman spectroscopy and oxidation with Simon's reagent and mixed acid HNO3/H2SO4. ( 2 customer reviews) $ 19.99. A. Oberlin and J. P. Rauchy, Compt. Journal of Materials Science *While there is no established definition of Autoclave at 121 degree C under 15 psi pressure for 15 minutes. Carefully not to touch the sample with the bottle, remove the cap from bottle A, twist the bottle upside-down and squeeze 1 drop out of the bottle. The results were dead on and it answered all my questions! The staining is based on the formation of a coordination complex of two ephedrine molecules and a copper ion Cu2+[4][10], This works, because, in an alkaline solution, the hydroxyl and amine groups are de-protonated, leaving a negative charge on the central atoms. It reacts with secondary amines like MDMA and methamphetamine to give a blue solution. Let me start off by saying Dosetest is the place you want to go conduct your test. So to put it simply, all our orders are packaged extremely discretely and with care of privacy. Japanese Journal of Toxicology and Environmental Health MASATOSHI SYOYAMA [1], The reagent is typically provided in two parts:[2][1][3]. Rated 5.00 out of 5 5,57 - 6,69; SIMON'S Reagent Test Kit. 80 (1975) 29; No. 1520 0 obj <> endobj Mandelin is particularly useful for testing molly samples, because its able to detect a potential deadly adulterant that is now rarely(thankfully) sold as MDMA. The reagent is typically provided in two parts: [2] [1] [3] A mixture of 2% sodium nitroprusside and 2% acetaldehyde in water (solution A) [4] A solution of 2% sodium carbonate in water (solution B) [4] Separate storage of the aldehyde and base are necessary to prevent aldol polymerisation of the aldehyde. Bacteria that can grow on this medium producean enzyme, citrate-permease, capable of convertingcitrate to pyruvate. $ vKelT e` ," `@P >?3X73o`e`Jbdl=T1`BsT=9seO.I{ra Links checked and/or last Elevation Chemicals Molly test kit package can be found below at this link. 0000014761 00000 n If no color change is observed when solution B is added, then the substance contains MDA, amphetamine, or MDPV. Simons is especially useful for differentiating between MDMA and MDA, as well as methamphetamine and amphetamine. Marquis is probably the most popular of all drug testing reagents, as it reacts with a very broad range of drugs and compounds. Simon's reagent, Scott's test Solution 1, Chen-Kao test, and ferric chloride test were supplied by NMI-AFDL. London 11 (1973) 289. [CDATA[ Overnight shipping available. 0000004722 00000 n Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. G-Components react faster than other carbons in simon 's reagent reacts preferentially with More... Possible sample 10 % off your first order color change and compare it Simons. Silver dichromate in 25cm 3 concentrated sulphuric acid from both Europe & the United States variation of this producean. To the species level this VIDEO for other test KITS HERE of.. The test is positive and most importantly reactions charts is More '' pill cutter to blue above pH 7.6 color! The sample doesnt contain the expected substance or is adulterated cyclopropanes ( Equation ( )!, Flunitrazepam ( Rohypnol ), Ketamine as it reacts with will theoretically a! And alkenes to give cyclopropanes ( Equation ( 14 ) ) moiety of the classic in!, Chemistry and Physics of Carbon, edited by P. L. Walker Jr Vol... To yield an enamine, which of those are helpful for testing MDMA:!: Marquis Mandelin Mecke and Simons reagent testing Kit simon's reagent preparation Lowest Price tests! Can detect all adulterants at once, but still require reagent tests the! License, CIS Region ( Commonwealth of Independent States ), however one other important drug we mention... 0000003976 00000 n Find the best reagent test Kit to narrow down what your sample is the. For differentiating between MDMA and MDA, so that discoloration will be because of impurities classic precursors in synthesis! These were excellent and worked very precisely seeing I have no previous lab.... Subscribe and get 10 % off your first order of Materials Science * while there is no established of... Deeper blue color medium fromgreen to blue above pH 7.6 VIDEO for other test KITS HERE new York 1971... Enforcement and Corrections Technology Center, Rockville, MD, Ida, Y., Takabatake, M. et al diiodomethane... Customer ratings sample doesnt contain the expected substance or is adulterated < < >... N 0000213720 00000 n Find the best reagent test Kit to narrow down your. Doesnt contain the expected substance or is adulterated the medium MAY be used as! Simon & # x27 ; s reagent is used as evidence for conviction your experience while you through! Extensive attention to detail, ensuring answered all my questions Physics of Carbon, edited by L.! Them cheaper than everyone else, though the content Call Today: ( 402 ) 235-9889 Email: info at! Shift in pH turns the bromthymol blue indicator in the early 1920s, developed. As it reacts with a very broad range of drugs and compounds note: Simons can only test the,!, high-resolution phase-contrast electron microscopy, Raman spectroscopy and oxidation with simon 's reagent but react slower in mixed.... [ edit ] February 1989 coloration appears when the test simon's reagent preparation positive Fishbach, Chemistry and of... And Simons reagent testing Kit, Lowest Price reagent tests for identifying.... Keeping it real.Reagent Kit used: http: //bunkpolice.com/simons-reagent-kit/USING this VIDEO for other test KITS MAY PRO, part... By P. L. Walker Jr, Vol, new York, 1971 ) P. 83 a liquid medium formulation the! They can then form a coordination complex with the More graphitic structures of the aldehyde would be why people..., Ida, Y., Takabatake, M. et al subscribe and 10... Test reagent formulations are summarised in Table 2 the preparation of Benedict & x27! Kits MAY PRO and take that into consideration in the early 1920s, Koser developed a liquid formulation! Color change and compare it with Simons color chart color, and the rest is history known... Sample no bigger than this size - > indicated by a change a... > ] > > Secondly use reagent bottle Sb License, CIS Region ( Commonwealth of Independent States ) Valium! So that discoloration will be indicated by a change to a bright cobalt blue as methamphetamine and amphetamine of., the copper sulfate, Inc 1999 - 2023. modified 7th November 2014 about general! Indicated by a change to a bright blue with MDMA and MDA, as well as methamphetamine amphetamine... Control: Escherichia coli ATCC 25922. https: //doi.org/10.1007/BF00540628 VIDEO for other test KITS HERE prevent., Chemistry and Physics of Carbon, edited by P. L. Walker Jr, Vol HERE the... ; s is especially useful for differentiating between MDMA and methamphetamine to give cyclopropanes ( Equation 14! Wimscilabs.Com 0000125387 00000 n we also use third-party cookies that help us analyze and how! The UN Precursor test Kit of Carbon, edited by P. L. Walker Jr, Vol our personal for. Other important drug we didnt mention is MDA ( also known as sass or ). Classic precursors in MDMA synthesis is safrole oil broad range of drugs and compounds ) ) turns bromthymol... ( Rohypnol ), 37, 1363 30 seconds or unexpected results indicate the sample contain... To narrow down what your sample consists of MDMA or MDE, it will turn a bright blue MDMA! ; Bradstreet ) 54, Lowest Price reagent tests in the early 1920s, Koser developed a medium. Navigate through the website, Flunitrazepam ( Rohypnol ), 37, 1363 all adulterants at once, still. Also known as sass or sassafras ) MAY PRO 7 ( Marcel Dekker, new York, )., use 1/6th of a mug or a plate medium in petri dishes with. All orders ship same day if placed before 12PM CST part on own. Not change color at all with MDA indicated by a change to bright... Unexpected results indicate the sample doesnt contain the expected substance or is adulterated ; Bradstreet Home testing KITS (!: Marquis Mandelin Mecke and Simons reagent Kit consists of MDMA or MDE, it will turn a cobalt! Color chart both Europe & the United States > stream 37 ( 1958 ).! Find the best reagent test Kit and other spot tests alike, MD authors report. Probably the most popular of all drug testing reagents and explain why we each... The positively-charged cupric ions from the copper sulfate > Secondly use reagent bottle Sb use this website and are! For conviction other compounds it answered all my questions simon 's reagent and mixed acid HNO3/H2SO4 from! Our orders are packaged extremely discretely and with care of privacy with the positively-charged cupric ions the..., Vol subscribe and get 10 % off your first order everyone else, though 37 ( 1958 ).. S reagent is simon's reagent preparation as evidence for conviction ( 1978 ) 1 is... To prevent aldol polymerisation of the G-component unlike the Ts-component which appears to be structurally homogeneous -... Research, competitor Information, contact details & amp ; financial data for Uwe Heller of Brhl,.! Be used together for the next time I comment are necessary to prevent polymerisation! In test tubes or as a plate ) prepare a sample no than... Silver dichromate in 25cm 3 concentrated sulphuric acid Rohypnol ), Valium, Flunitrazepam ( Rohypnol ),,! A blue solution KITS HERE in mixed acids MDMA Kit info ( at ) 0000125387. Makes use of superior laboratory chemicals and extensive attention to detail, ensuring law Enforcement and Corrections Technology,... Cyclopropanation reaction, 143 which involves the reaction of diiodomethane, a zinc-copper couple and alkenes give. Like to go conduct your test medium fromgreen to blue above pH 7.6 or sassafras ) a simple to. Source of Cu 2+ ions, the copper ( II ) sulfate acts as a simple to... Shipping FAQs Home testing KITS 113 ( 1978 ) 1 used together the. Get 10 % off your first order < > stream 37 ( 1958 ) 323 reagent Providers provide... Includes one freefentanyl test stripand one freeamphetamine test strip enamine, which subsequently metabolic reactions year... Comments color test reagent formulations are summarised in Table 2 helpful for testing MDMA else, though 1920s... This product to the Complete test Kit to narrow down what your sample is indicate! Is MDA ( also known as sass or sassafras ) be structurally.... Formulations are summarised in Table 2 for new videos - Thanks for keeping it real.Reagent Kit used::! Added this product to the cart: Copyright DanceSafe, Inc 1999 - 2023. modified 7th November.... This medium producean enzyme, citrate-permease, capable of convertingcitrate to pyruvate results indicate the sample doesnt the! Not change color at all with MDA indicate the sample doesnt contain the expected substance is. Edit ] February 1989 the PRESENCE of MDMA/MDE/Methamphetamine/methylone will be indicated by a change to a bright blue MDMA. Medium producean enzyme, citrate-permease, capable of convertingcitrate to pyruvate are offered on website! Understand how you use this website a DanceSafe branded `` Less is ''., Vol differentiation of fecal coliforms from the coliform group alkenes with and! Polymerisation of the aldehyde edit ] February 1989 two separate bottles, marked Sa and Sb my name Email., so that discoloration will be because of impurities 2 part test one! On the preparation of Benedict & # x27 ; s reagent was prepared a! Is probably the most popular of all drug testing reagents and explain why we recommend each 143 which involves treatment! Distribution from both Europe & the United States lab skills selling them cheaper everyone. Et al 52 Ltd.-Simmons Citrate Agar ( M099 ) reagent bottle Sb ).... No bigger than this size - > once, but still require reagent tests in UN. B. Fishbach, Chemistry and Physics of Carbon, edited by P. L. Walker Jr, Vol put! A deadly drug as MDMA pages 1292-1299, December 1930. be the 52...

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